Thyme phenol has antibacterial and antioxidant activities

7956721

Thymol (5-methyl-2-propofol), also known as thymol, is an aromatic compound that has the characteristic aroma of thyme or thyme and is found in the essential oils of many plants.Although thymol has good antibacterial activity and antioxidant property, its application in food is limited by its difficulty in dispersing well in aqueous phase system.Microemulsification is a method for dispersing lipid-soluble substances evenly in aqueous systems.In this study, the water solubility of thymol was improved by constructing a microemulsion system to transport thymol.With the aid of quasi-ternary phase diagram, different non-ionic surfactants and anionic and cationic surfactants were selected to test and select various components of the microemulsion and to construct a microemulsion system with thymol as oil phase.Differential calorimetry scanning, dynamic laser scattering and the changes of conductivity and viscosity were used to analyze the microstructure evolution of the microemulsion system.When the water content of the system is 0-20%, the system is in the W/O stage.When the water content is 25-35%, the system is bicontinuous.When the water content is 40-90%, the system is an O/W structure.The results of conductivity measurement are consistent with the results of DSC analysis.The results of viscosity determination showed that the microemulsion particles underwent a transformation from hexagonal or rod-shaped micelles to spherical micelles in the O/W range.

The bacteriostatic activity of different kinds of microemulsion systems against typical foodborne pathogens was studied by microbial lethal kinetics experiment.The results showed that with the increase of water content in the system, the inhibitory effect of the microemulsion system gradually decreased, and the oil-in-water system showed a good bacteria-inhibiting effect on gram-positive and gram-negative bacteria, while the oil-in-water system had a relatively weak bacteria-inhibiting effect.In addition, the antioxidant activity of thymol microemulsion system with different water content was investigated by DPPH free radical scavenging experiment.It was found that the free radical scavenging ability of the microemulsion system increased with the increase of water content. Although more thymol was embedded in W/O microemulsion, the contact between DPPH and thymol was limited by the high concentration of surfactants.Although the content of thymol in O/W microemulsion is low, the reaction interface is large and the free radical scavenging ability is good.

Because of surfactant in the food system usage need to control in low concentration, so the the lower concentration of surfactant surfactant of thyme phenolic solubilization effect, and thyme of phenol by 1 h nuclear magnetic resonance (NMR) analysis in 80, the distribution situation of micelle, twain by DPPH radical scavenging test and FRAP method for determining the thyme of phenol twain 80 micellar solution antioxidant function.The results showed that the maximum increased solution of thymol in 2.0% tween 80 micelle solution was 0.2%.1 h nuclear magnetic resonance (NMR) results the thyme of phenol in micellar distribution location close to the head of polyoxyethylene base connected with oleic acid ester base, DPPH radical scavenging ability and FRAP method to determine the total reducing power showed a similar trend, antioxidant pecking order is: butylated hydroxytoluene > thyme phenol twain 80 micellar solution > phenol, thyme thyme solubilization of phenol to improve their antioxidant activity, and its antioxidant ability and synthetic antioxidant butylated hydroxytoluene.